My fascination with toxicology centers on the elusive relationship between environmental contaminants and disease. Many diseases are suspected of having environmental causes; however, many of the causes and their molecular mechanisms are not well understood. Research in the area is key to the prevention of disease and vital to the status of human health.
Thesis Title: Towards Absolute Quantitation in Cell Culture: Expression of Dose of Xenobiotics and Capacity of Cells to Remove H2O2 with Implications to Pharmacological Ascorbate in Cancer Therapy
Advisor: Garry Buettner, Ph.D.
Year of Graduation: 2016
Current Position: Mammalian Toxicology Consultant
Current Employer/Institution: Dow Chemical
Publications from Human Toxicology Program Research:
van 't Erve TJ, Doskey CM, Wagner BA, Hess JR, Darbro BW, Ryckman KK, Murray JC, Raife TJ, Buettner GR. (2014) The heritability of glutathione and related metabolites in stored red blood cells. Free Radic Biol Med. 76,107-113, PMID: 25108189, PMCID: PMC4252477.
Doskey CM, van 't Erve TJ, Wagner BA, Buettner GR. Moles of a Substance per Cell Is a Highly Informative Dosing Metric in Cell Culture. PLoS One. 10, e0132572 (2015), PMID: 2617283, PMC4501792.
*Xiao W, Sarsour EH, Wagner BA, Doskey CM, Buettner GR, Domann FE, Goswami PC. Succinate dehydrogenase activity regulates PCB3-quinone-induced metabolic oxidative stress and toxicity in HaCaT human keratinocytes. Arch Toxicol. 2014 Nov 23. PMID: 25417049, PMCID: PMC4441874.
Claire M Doskey, Visarut Buranasudja, Brett A Wagner, Justin G Wilkes, Juan Du, Joseph J Cullen, Garry R Buettner, Tumor cells have decreased ability to metabolize H 2 O 2: Implications for pharmacological ascorbate in cancer therapy, Redox Biol, 2016 Dec;10:274-284, doi: 10.1016/j.redox.2016.10.010.
Visarut Buranasudja, Claire M Doskey, Adrienne R Gibson, Brett A Wagner, Juan Du, David J Gordon, Stacia L Koppenhafer, Joseph J Cullen, Garry R Buettner, Pharmacologic Ascorbate Primes Pancreatic Cancer Cells for Death by Rewiring Cellular Energetics and Inducing DNA Damage, Mol Cancer Res, 2019 Oct;17(10):2102-2114, doi: 10.1158/1541-7786.MCR-19-0381